Figure 2
From: Virtual cooperativity in myoglobin oxygen saturation curve in skeletal muscle in vivo
Steady-state O2 uptake rate (V) of perfused rat hindlimb muscles as a function of effluent buffer PO2. The rat hindlimb was perfused with Krebs-bicarbonate buffers containing no additive (●) as control, 0.4 mM of KCN (○) for suppression of muscle respiration, and 5 μM (△) or 10 μM (▲) 2,4-dinitrophenol for stimulation of muscle respiration. Symbols express observed points. Each plot is the mean of experiments using three animals, and the errors for each data point are less than the size of symbols. The solid lines were calculated using a rectangular hyperbolic curve: V = Vmax(PO2/PV50)/{1 + (PO2/PV50)}. The values of PV50 and Vmax are given in Table 1 which also includes the maximal values of influent and effluent PO2.